A case study of pneumonic changes and death in Nilgai (Boselaphus tragocamelus) with respiratory disease in Punjab, Pakistan

Sabeera Siddiq; Khawar Ali Shahzad

doi:10.47264/idea.nasij/4.1.9

Authors

Sabeera Siddiq Department of Zoology, The Islamia University of Bahawalpur, Bahawalpur, Pakistan. https://orcid.org/0009-0008-0205-0454
Khawar Ali Shahzad Shanghai Fourth People’s Hospital, and School of Medicine, Tongji University, Shanghai, China. https://orcid.org/0000-0003-3798-5301

DOI:

https://doi.org/10.47264/idea.nasij/4.1.9

Keywords:

Nilgai, hepatization, lesions, necropsy, pathogens, pneumonia, ulceration, respiratory disease, bovine respiratory disease

Abstract

A nine-year-old Nilgai (Boselaphus tragocamelus) in Bahawalpur Zoo, Punjab, Pakistan, was ill with respiratory disease, so the pathogens involved were studied, and morphological features and propensity for Bovine Respiratory Disease (BRD) involved were also speculated. Because of demolition, Nilgai is sparsely populated in Pakistan, so studying the cause of its death was essential. The lung samples were collected, and histopathological techniques and bacterium isolation were used to analyse them. Necropsy, i.e., gross and histopathological postmortem exams, were carried out in the laboratory using customary methods under rigorous crafting circumstances. A standard visual and microscopic methodology was used in gross examination, and routine microbiologic tests were run. Specific and compelling infectious agents have been found in Nilgai's lungs, including Pasteurella multocida and Mycoplasma bovis. Pathology and histopathology revealed that Nilgai had extensive ulceration and chronic swelling behind his mandibles, and the post-mortem report verified this. The report revealed that the lungs were grey hepatised. Histological lesions represented acute, severe, haemorrhagic, and chronic bronchopneumonia stages of pneumonia. Lungs with histological lesions were classified as pattern pneumonia. The current study's goals were to report clinical, pathologic, and molecular data and to determine the cause of the respiratory ailment that affected one Nilgai.

References

Abdeen, E. E., Mousa, W. S., & Suelam, I. I. (2017). Genotyping of Mycoplasma bovis isolated from cattle suffering from respiratory manifestation in Menofia province, Egypt. Pak. Vet. J, 37(1), 69-72. https://doi.org/10.22207/JPAM.12.4.47 DOI: https://doi.org/10.22207/JPAM.12.4.47

Bancroft, J. D., & Gamble, M. (2008). Theory and practice of histological techniques. (6th Edition). Churchill Livingstone, Elsevier.

Belkhiri, M., Tlidjane, M., Benhathat, Y., & Meziane, T. (2009). Histopathological study and pulmonary classification of bovine lesions. African Journal of Agricultural Research, 4(7), 584-591. https://doi.org/10.18535/jmscr/v5i12.15 DOI: https://doi.org/10.18535/jmscr/v5i12.15

Buczinski, S., & Pardon, B. (2020). Bovine respiratory disease diagnosis: What progress has been made in clinical diagnosis? Veterinary Clinics: Food Animal Practice, 36(2), 399-423. https://doi.org/10.1016/j.cvfa.2020.03.004 DOI: https://doi.org/10.1016/j.cvfa.2020.03.004

Caswell, J. L., Bateman, K. G., Cai, H. Y., & Castillo-Alcala, F. (2010). Mycoplasma bovis in respiratory disease of feedlot cattle. Veterinary Clinics: Food Animal Practice, 26(2), 365-379. https://doi.org/10.1016/j.cvfa.2010.03.003 DOI: https://doi.org/10.1016/j.cvfa.2010.03.003

Chauhan, V., Mahesh, D., Panda, P., Mokta, J., & Thakur, S. (2010). Leptospirosis presenting as acute respiratory distress syndrome (ARDS) in sub-Himalayan region. J Assoc Physicians India, 58, 390-391. https://doi.org/10.4103/0255-0857.188370 DOI: https://doi.org/10.4103/0255-0857.188370

Dhara, A., Saini, M., Das, D. K., Swarup, D., Sharma, B., Kumar, S., & Gupta, P. K. (2007). Molecular characterization of coding sequences and analysis of Toll-like receptor 3 mRNA expression in water buffalo (Bubalus bubalis) and Nilgai (Boselaphus tragocamelus). Immunogenetics, 59, 69-76. https://doi.org/10.1007/s00251-006-0174-6 DOI: https://doi.org/10.1007/s00251-006-0174-6

Dudley, M. A., & Willett, H. P. (1965). Nicotinamide Adenine Dinucleotide Synthesis by Mycobacterium tuberculosis van bovis. Proceedings of the Society for Experimental Biology and Medicine, 119(3), 807-812. https://doi.org/10.1139/m66-164 DOI: https://doi.org/10.3181/00379727-119-30307

Earley, B., Sporer, K. B., & Gupta, S. (2017). Invited review: Relationship between cattle transport, immunity and respiratory disease. Animal, 11(3), 486-492. https://doi.org/10.1017/S1751731116001622 DOI: https://doi.org/10.1017/S1751731116001622

Gallagher, D., Davis, S., De Donato, M., Burzlaff, J., Womack, J., Taylor, J., & Kumamoto, A. (1998). A karyotypic analysis of nilgai, Boselaphus tragocamelus (Artiodactyla: Bovidae). Chromosome Research, 6, 505-514. https://doi.org/10.1894/0038-4909-64-2-145 DOI: https://doi.org/10.1023/A:1009268917856

Gourlay, R., Mackenzie, A., & Cooper, J. (1970). Studies of the microbiology and pathology of pneumonic lungs of calves. Journal of Comparative Pathology, 80(4), IN575-IN511. https://doi.org/10.1016/0021-9975(70)90056-3 DOI: https://doi.org/10.1016/0021-9975(70)90055-1

Holman, D. B., Timsit, E., Amat, S., Abbott, D. W., Buret, A. G., & Alexander, T. W. (2017). The nasopharyngeal microbiota of beef cattle before and after transport to a feedlot. BMC Microbiology, 17, 1-12. https://doi.org/10.2527/asasann.2017.060 DOI: https://doi.org/10.1186/s12866-017-0978-6

Islam, E. A., Fegan, J. E., Tefera, T. A., Curran, D. M., Waeckerlin, R. C., Ng, D., . . . Shah, M. (2023). Reverse vaccinology-based identification of a novel surface lipoprotein that is an effective vaccine antigen against bovine infections caused by Pasteurella multocida. PLoS Pathogens, 19(3), e1011249. https://doi.org/10.1371/journal.ppat.1011249 DOI: https://doi.org/10.1371/journal.ppat.1011249

Johnson, K. K., & Pendell, D. L. (2017). Market impacts of reducing the prevalence of bovine respiratory disease in United States beef cattle feedlots. Frontiers in Veterinary Science, 4, 189. https://doi.org/10.3389/fvets.2017.00189 DOI: https://doi.org/10.3389/fvets.2017.00189

Lendrum, A., Fraser, D., Slidders, W., & Henderson, R. (1962). Studies on the character and staining of fibrin. Journal of Clinical Pathology, 15(5), 401. https://doi.org/10.1136/jcp.25.5.373 DOI: https://doi.org/10.1136/jcp.15.5.401

Leslie, D. M., & Schaller, G. B. (2008). Pantholops hodgsonii (Artiodactyla: Bovidae). Mammalian Species, (817), 1-13. https://doi.org/10.1644/817.1 DOI: https://doi.org/10.1644/817.1

Madsen, T., Arnal, A., Vittecoq, M., Bernex, F., Abadie, J., Labrut, S., . . . Beckmann, C. (2017). Cancer prevalence and etiology in wild and captive animals. In B. Ujvari, B. Roche, & F. Thomas (eds.), Ecology and evolution of cancer (pp. 11-46). Elsevier. DOI: https://doi.org/10.1016/B978-0-12-804310-3.00002-8

Marie, E. B. (2016). Pathological pulmonary lesions in cows in Mosul Province in 2013. Journal of Health, Medicine and Nursing, 29, 53-59. https://core.ac.uk/download/pdf/234691878.pdf

Munir, M. (2014). Role of wild small ruminants in the epidemiology of peste des petits ruminants. Transboundary and Emerging Diseases, 61(5), 411-424. https://doi.org/10.1111/tbed.12052 DOI: https://doi.org/10.1111/tbed.12052

Oguya, B., & Eltringham, S. (1991). Behaviour of nilgai (Boselaphus tragocamelus) antelope in captivity. Journal of Zoology, 223(1), 91-102. https://doi.org/10.9734/ajriz/2022/v5i130128 DOI: https://doi.org/10.1111/j.1469-7998.1991.tb04751.x

Ollivett, T. L., & Buczinski, S. (2016). On-farm use of ultrasonography for bovine respiratory disease. Veterinary Clinics: Food Animal Practice, 32(1), 19-35. https://doi.org/10.1016/j.cvfa.2015.09.001 DOI: https://doi.org/10.1016/j.cvfa.2015.09.001

Pa?nik, M., Bestry, I., & Roszkowski-?li?, K. (2017). Solitary pulmonary nodule—the role of imaging in the diagnostic process. Advances in Respiratory Medicine, 85(6), 345-351. https://doi.org/10.5603/arm.2017.0059 DOI: https://doi.org/10.5603/ARM.2017.0059

Rafferty, J. P. (2010). Grazers: Britannica Educational Publishing.

Ranjan, R., Narnaware, S. D., Nath, K., Sawal, R., & Patil, N. (2018). Rapid diagnosis of tuberculosis in dromedary camel (Camelus dromedarius) using lateral flow assay-based kit. Tropical Animal Health and Production, 50, 907-910. https://doi.org/10.1007/s11250-017-1502-6 DOI: https://doi.org/10.1007/s11250-017-1502-6

Rezac, D., Thomson, D., Bartle, S., Osterstock, J., Prouty, F., & Reinhardt, C. (2014). Prevalence, severity, and relationships of lung lesions, liver abnormalities, and rumen health scores measured at slaughter in beef cattle. Journal of Animal Science, 92(6), 2595-2602. https://doi.org/10.2527/jas.2013-7222 DOI: https://doi.org/10.2527/jas.2013-7222

Schmidly, D. J., & Bradley, R. D. (2016). The mammals of Texas. University of Texas. DOI: https://doi.org/10.7560/308868

Sheffield, W. J. (1983). Food habits of nilgai antelope in Texas. Journal of Range Management, 36(3), 316-322. https://repository.arizona.edu/handle/10150/645878 DOI: https://doi.org/10.2307/3898478

Singh, P., Gopalaswamy, A. M., & Karanth, K. U. (2010). Factors influencing densities of striped hyenas (Hyaena hyaena) in arid regions of India. Journal of Mammalogy, 91(5), 1152-1159. https://doi.org/10.1644/09-mamm-a-159.1 DOI: https://doi.org/10.1644/09-MAMM-A-159.1

Swai, E., Mwezimpya, I., Ulicky, E., Mbise, A., & Moshy, W. (2013). An abattoir survey of contagious bovine pleuropneumonia lesions in slaughtered cattle in selected districts in Northern Tanzania. Asian Pacific Journal of Tropical Biomedicine, 3(4), 303-306. https://doi.org/10.1016/s2221-1691(13)60067-2 DOI: https://doi.org/10.1016/S2221-1691(13)60067-2

Szczyrek, M., Krawczyk, P., Milanowski, J., Jastrzebska, I., Zwolak, A., & Daniluk, J. (2011). Chronic obstructive pulmonary disease in farmers and agricultural workers-an overview. Annals of Agricultural and Environmental Medicine, 18(2). https://doi.org/10.26444/aaem/74651 DOI: https://doi.org/10.26444/aaem/74651

Taylor, J. D., Fulton, R. W., Lehenbauer, T. W., Step, D. L., & Confer, A. W. (2010). The epidemiology of bovine respiratory disease: What is the evidence for predisposing factors? The Canadian Veterinary Journal, 51(10), 1095. https://doi.org/10.21423/aabppro20084460 DOI: https://doi.org/10.21423/aabppro20084460

Tsegaye, S., Tessema, D., & Asebe, G. (2016). Gross Pulmonary Lesions of Bovine Lung Slaughtered at Jimma Municipality Abattoir, Ethiopia. J. Veterinar Sci Technol, 7(356), 2. https://doi.org/10.4172/2157-7579.1000356 DOI: https://doi.org/10.4172/2157-7579.1000356

van Dijk, C. E., Zock, J.-P., Baliatsas, C., Smit, L. A., Borlée, F., Spreeuwenberg, P., . . . Yzermans, C. J. (2017). Health conditions in rural areas with high livestock density: Analysis of seven consecutive years. Environmental Pollution, 222, 374-382. https://doi.org/10.1016/j.envpol.2016.12.023 DOI: https://doi.org/10.1016/j.envpol.2016.12.023

Verma, R., Sharma, A., Sangram, P. R., Kidangam, A., Mandai, T., & Verma, H. (2012). Generalized tuberculosis in Nilgai (Boselaphus tragocamelus) caused by Mycobacterium fortuitum. https://www.indianjournals.com/ijor.aspx?target=ijor:ijvp&volume=36&issue=1&article=008

Worku, A., Abreham, S., Hailu, M., Mamo, G., Ameni, G., & Tsegaye, S. (2016). Cross-sectional study and comparison of different diagnostic methods of bovine tuberculosis in gondar elfora abattoir, Ethiopia. Mycobact Dis, 6(218), 2161-1068.1000218. https://doi.org/10.4172/2161-1068.1000218 DOI: https://doi.org/10.4172/2161-1068.1000218

Zeryehun, T., & Alemu, B. (2017). Major gross lesions of lung in cattle slaughtered at hawassa municipal abattoir, Southern Ethiopia. Journal of Veterinary Medicine, 2017. https://doi.org/10.1155/2017/1702852 DOI: https://doi.org/10.1155/2017/1702852